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Animal Transgenesis and Cloning.
Louis-Marie Houdebine
Copyright ¶ 2003 John Wiley & Sons, Ltd.
ISBNs: 0-470-84827-8 (HB); 0-470-84828-6 (PB)
Animal Transgenesis
and Cloning
Animal Transgenesis
and Cloning
Louis-Marie Houdebine
Institut National de la Recherche Agronomique,
Jouy en Josas, France
Translated by
Louis-Marie Houdebine, Christine Young,
Gail Wagman and Kirsteen Lynch
First published in French as TransgenÁse Animale et Clonage # 2001 Dunod, Paris
Translated into English by Louis-Marie Houdebine, Christine Young, Gail Wagman and
Kirsteen Lynch.
This work has been published with the help of the French Minist
Á
re de la Culture-Centre
national du livre
English language translation copyright # 2003 by John Wiley & Sons Ltd,
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ISBN 0-470-84827-8 (Hardback)
0-470-84828-6 (Paperback)
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Contents
Introduction
ix
Abbreviations and Acronyms
xiii
1 From the gene to the transgenic animal
1
1.1 Genome composition
1
1.2 Gene structure
4
1.3 The number of genes in genomes
7
1.4 The major techniques of genetic engineering
13
1.4.1 Gene cloning
13
1.4.2 DNA sequencing
14
1.4.3 In vitro gene amplification
14
1.4.4 Gene construction
14
1.4.5 Gene transfer into cells
16
1.5 The systematic description of genomes
21
1.6 Classical genetic selection
26
1.7 Experimental mutation in genomes
27
1.7.1 Chemical mutagenesis
27
1.7.2 Mutagenesis by integration of foreign DNA
29
1.7.3 Mutagenesis by transgenesis
30
2 Techniques for cloning and transgenesis
33
2.1 Cloning
33
2.1.1 The main steps of differentiation
33
2.1.2 Cloning by nuclear transfer
37
2.2 Gene therapy
48
2.2.1 The goals of gene therapy
48
2.2.2 The tools of gene therapy
49
2.2.3 The applications of gene therapy
52
2.3 Techniques of animal transgenesis
54
2.3.1 The aims and the concept of animal transgenesis
54
2.3.2 Gene transfer into gametes
60
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